Single molecule analyses in complex matrices

Quantitative analysis of metabolites and macromolecules using a variety of techniques is a cornerstone of our research. We continue a proud tradition of single molecule extraction ‘the hard way’ using extraction by solvent or supercritical fluid, group separation by ion exchange or hydrophobic interaction, and separation & quantification by HPLC, capillary electrophoresis, or supercritical fluid chromatography. The analytical techniques are backed up by isolation and structure elucidation by spectroscopy.

 

The classical approach is supplemented by advanced modern ‘shortcuts’, in particular immunochemical methods for quantification of both small molecules and macromolecules. Not only are the latter methods quick, they also allow analyses that would be highly demanding or impossible by the classical methods based on separation.

 

 

 
Selected recent papers:

 

Niels Agerbirk, Carl Erik Olsen, Eva Poulsen, Niels Jacobsen, Paul Robert Hansen, 2010. Complex metabolism of aromatic glucosinolates in Pieris rapae caterpillars involving nitrile formation, hydroxylation, demethylation, sulfation, and host plant dependent carboxylic acid formation. Insect Biochemistry and Molecular Biology In press.

 

Skottrup, P., Frøkiær, H., Hearty, S., O’Kennedy, R., Hejgaard, J., Nicolaisen, M. & A.F. Justesen (2007): Monoclonal antibodies for Puccinia striiformis uredinospore detection, Mycol. Res. 117:332-338.

 

Petersen, I.L., Hansen, H.C.B, Ravn, H.W, Sørensen, J.C. Sørensen, H.: Metabolic effects in rapeseed (Brassica napus L.) seedlings after root exposure to glyphosate. Pesticide Biochemistry and Physiology (2007) 89, 220-229.

 

Knudsen, D L, Røn, Ø, Baardsen, G., Smedsgaard, J, Koppe, W & H Frøkiær (2006): Soybean saponins resist extrusion cooking and are indigestible for Atlantic salmon (Salmo salar L.). J. Agric. Food Chem. 54:6428-6435.

 

Bellostas, N., Sørensen, J.C. and Sørensen, H.: Micellar electrokinetic capillary chromatography – Synchronous monitoring of substrate and products in the myrosinase catalysed hydrolysis of glucosinolates. J. Chromatogr. A. (2006), 1130, 246-252.

 

Andersen, K.E., Bjergegaard, C., Møller, P., Sørensen, J.C., and Sørensen, H. 2005. Compositional variations for α-galactosides in different species of Leguminosae and Brassicaceae – A chemotaxonomic study based on chemometrics and high performance capillary electrophoresis with indirect UV detection. J. Agric. Food Chem., 53:5809-5817.